Annular impinging jet with recirculation zone expanded by acoustic excitation

Flow visualization and mass transfer (naphthalene sublimation) experiments were performed on acoustically excited annular air jet with diameter ratio Di/Do=0.95. Two different regimes of the time-mean flow field were found, differing in the size of the central recirculation zone, with either the single stagnation point or the stagnation circle. The switching between the two regimes is accomplished by acoustic excitation, under identical geometry conditions. An effective stabilization of the large recirculation zone, as well as remarkable augmentation of average heat/mass transfer by 23%, have been achieved at the excitation Strouhal number Sh=0.94

Enhancement of synthetic jets by means of an integrated valve-less pump Part II. Numerical and experimental studies

The paper studies the performance of the new fluid jet actuator based on the novel principle of the generation of fluid jet, which has been presented in [Z. Travnicek, A.I. Fedorchenko, A.-B. Wang, Enhancement of synthetic jets by means of an integrated valve-less fluid pump. Part I. Design of the actuator, Sens. Actuators A, 120 (2005) 232-240]. The fluid jet actuator consists of a synthetic jet actuator and a valve-less pump. The resulting periodical fluid jet is intrinsically non-zero-net-mass-flux, in contrast to the traditional synthetic jet. The numerical results have been compared with the laboratory experiments comprising phase-locked smoke visualization and time-mean velocity measurements. The results have confirmed the satisfactory performance of the actuator

No-moving-part hybrid-synthetic jet actuator

In contrast to usual synthetic jets, the “hybrid-synthetic jets” of non-zero timemean nozzle mass flow rate are increasingly often considered for control of flow separation and/or transition to turbulence as well as heat and mass transfer. The paper describes tests of a scaled-up laboratory model of a new actuator version, generating the hybrid-synthetic jets without any moving components. Self-excited flow oscillation is produced by aerodynamic instability in fixed-wall cavities. The return flow in the exit nozzles is generated by jet-pumping effect. Elimination of the delicate and easily damaged moving parts in the actuator simplifies its manufacture and assembly. Operating frequency is adjusted by the length of feedback loop path. Laboratory investigations concentrated on the propagation processes taking place in the loop

Subdynamic asymptotic behavior of microfluidic valves

Decreasing the Reynolds number of microfluidic no-moving-part flow control valves considerably below the usual operating range leads to a distinct “subdynamic” regime of viscosity- dominated flow, usually entered through a clearly defined transition. In this regime, the dynamic effects on which the operation of large-scale no-moving-part fluidic valves is based, cease to be useful, but fluid may be driven through the valve (and any connected load) by an applied pressure difference, maintained by an external pressure regulator. Reynolds number ceases to characterize the valve operation, but the driving pressure effect is usefully characterized by a newly introduced dimensionless number and it is this parameter which determines the valve behavior. This summary paper presents information about the subdynamic regime using data (otherwise difficult to access) obtained for several recently developed flow control valves. The purely subdynamic regime is an extreme. Most present-day microfluidic valves are operated at higher Re, but the paper shows that the laws governing subdynamic flows provide relations useful as an asymptotic reference

Lupus nephritis management guidelines compared

In the past years, many (randomized) trials have been performed comparing the treatment strategies for lupus nephritis. In 2012, these data were incorporated in six different guidelines for treating lupus nephritis. These guidelines are European, American and internationally based, with one separate guideline for children. They offer information on different aspects of the management of lupus nephritis including induction and maintenance treatment of the different histological classes, adjunctive treatment, monitoring of the patient, definitions of response and relapse, indications for (repeat) renal biopsy, and additional challenges such as the presence of vascular complications, the pregnant SLE patient, treatment in children and adolescents and considerations about end-stage renal disease and transplantation. In this review, we summarize the guidelines, determine the common ground between them, highlight the differences and discuss recent literature

Towards a Robuster Interpretive Parsing

The input data to grammar learning algorithms often consist of overt forms that do not contain full structural descriptions. This lack of information may contribute to the failure of learning. Past work on Optimality Theory introduced Robust Interpretive Parsing (RIP) as a partial solution to this problem. We generalize RIP and suggest replacing the winner candidate with a weighted mean violation of the potential winner candidates. A Boltzmann distribution is introduced on the winner set, and the distribution’s parameter $T$ is gradually decreased. Finally, we show that GRIP, the Generalized Robust Interpretive Parsing Algorithm significantly improves the learning success rate in a model with standard constraints for metrical stress assignment

Implosive Thermal Plasma Source for Energy Conversion

Laboratory scale thermal plasma source for magnetohydrodynamic or magnetocumulative generator was developed. The thermal plasma is created from combustible stochiometric mixture of hydrogen and oxygen by spherical implosion of convergent detonation wave. Resulting high velocity plasma is observed by capturing emitted light by hi-speed camera to determine plasma velocity and also spectroscopically in order to estimate of plasma temperatures. Construction of the implosion plasma source is discussed

Comparison of FcRn- and pIgR-Mediated Transport in MDCK Cells by Fluorescence Confocal Microscopy

Protein delivery across polarized epithelia is controlled by receptor-mediated transcytosis. Many studies have examined basolateral-to-apical trafficking of polymeric IgA (pIgA) by the polymeric immunoglobulin receptor (pIgR). Less is known about apical-to-basolateral transcytosis, the direction the neonatal Fc receptor (FcRn) transports maternal IgGs across intestinal epithelia. To compare apical-to-basolateral and basolateral-to-apical transcytosis, we co-expressed FcRn and pIgR in Madin-Darby canine kidney (MDCK) cells and used pulse-chase experiments with confocal microscopy to examine transport of apically applied IgG Fcγ and basolaterally applied pIgA. Fcγ and pIgA trafficking routes were initially separate but intermixed at later chase times. Fcγ was first localized near the apical surface, but became more equally distributed across the cell, consistent with concomitant transcytosis and recycling. By contrast, pIgA transport was strongly unidirectional: pIgA shifted from near the basolateral surface to an apical location with increasing time. Some Fcγ and pIgA fluorescence colocalized in early (EEA1-positive), recycling (Rab11a-positive), and transferrin (Tf)-positive common/basolateral recycling endosomes. Fcγ became more enriched in Tf-positive endosomes with time, whereas pIgA was sorted from these compartments. Live-cell imaging revealed that vesicles containing Fcγ or pIgA shared similar mobility characteristics and were equivalently affected by depolymerizing microtubules, indicating that both trafficking routes depended to roughly the same extent on intact microtubules

The Chicken Yolk Sac IgY Receptor, a Mammalian Mannose Receptor Family Member, Transcytoses IgY across Polarized Epithelial Cells

In mammals the transfer of passive immunity from mother to young is mediated by the MHC-related receptor FcRn, which transports maternal IgG across epithelial cell barriers. In birds, maternal IgY in egg yolk is transferred across the yolk sac to passively immunize chicks during gestation and early independent life. The chicken yolk sac IgY receptor (FcRY) is the ortholog of the mammalian phospholipase A2 receptor, a mannose receptor family member, rather than an FcRn or MHC homolog. FcRn and FcRY both exhibit ligand binding at the acidic pH of endosomes and ligand release at the slightly basic pH of blood. Here we show that FcRY expressed in polarized mammalian epithelial cells functioned in endocytosis, bidirectional transcytosis, and recycling of chicken FcY/IgY. Confocal immunofluorescence studies demonstrated that IgY binding and endocytosis occurred at acidic but not basic pH, mimicking pH-dependent uptake of IgG by FcRn. Colocalization studies showed FcRY-mediated internalization via clathrin-coated pits and transport involving early and recycling endosomes. Disruption of microtubules partially inhibited apical-to-basolateral and basolateral-to-apical transcytosis, but not recycling, suggesting the use of different trafficking machinery. Our results represent the first cell biological evidence of functional equivalence between FcRY and FcRn and provide an intriguing example of how evolution can give rise to systems in which similar biological requirements in different species are satisfied utilizing distinct protein folds